Production of diosgenin from yellow ginger (<Emphasis Type="Italic">Dioscorea zingiberensis</Emphasis> C. H. Wright) saponins by commercial cellulase |
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Authors: | Wei Liu Wen Huang WeiLing Sun YuLing Zhu JinRen Ni |
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Institution: | (1) Department of Environmental Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences, Ministry of Education, 100871 Beijing, China;(2) Institute for Nanobiomedical Technology and Membrane Biology, State Key Laboratory of Biotherapy of Human Diseases, Cancer Center, West China Hospital, West China Medical School, Sichuan University, 610041 Chengdu, China; |
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Abstract: | A commercial cellulase was first assessed to be effective in hydrolyzing glycosyl at the C-3 and C-26 positions in steroidal
saponins from yellow ginger (Dioscorea zingiberensis C. H. Wright) to diosgenin, a very important chemical in the pharmaceutical industry. The effect of different parameters
on enzyme hydrolysis was further investigated by systematically varying them. The highest yield was achieved when the hydrolysis
ran at 55°C and pH 5.0 with an enzyme to substrate ratio of 15 × 103 U/g. The biotransformed products identified using TLC and HPLC confirmed that the cellulase was capable of releasing diosgenin
from steroidal saponins. Moreover, the biotransformation process was explored by LC-MS and LC-MS/MS analysis. Enzymatic hydrolysis
together with 40 % of the original sulphuric acid used increased the diosgenin yield by 15.4 ± 2.7% than traditional method.
Therefore, the commercial cellulase may serve as a promising tool for industrial diosgenin production and for further use
in saponin modification. |
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