A method for transient expression in maize endosperm |
| |
| Authors: | Deborah Vicuna Requesens Erin Egelkrout Shivakumar Devaiah Elizabeth E Hood |
| |
| Institution: | (1) Arkansas Biosciences Institute, Arkansas State University, P.O. Box 639, State University, AR 72467, USA;(2) Applied Biotechnology Institute, San Luis Obispo, CA, USA |
| |
| Abstract: | The ability to screen the functionality of gene constructs in a transient system of appropriate tissues informs the researcher
of the potential success of stable transformation. For this purpose, we developed a transient system to test the functionality
of endosperm-preferred promoters in maize. Two endosperm-preferred promoters from rice (a globulin and a glutelin promoter)
were employed. Ears of Zea mays L. were harvested at 17 d after pollination, surface sterilized and the endosperm excised. Using Agrobacterium tumefaciens co-cultivation and sonication, transient expression of the target genes was detected after 4 and 5 d. We demonstrate expression
of CBH I and CHB II (both exo-cellulases) up to 1.7% TSP, under the rice globulin and glutelin promoters. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
