The mouse Chromosome 7 distal imprinting domain maps to G-bands F4/F5 |
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Authors: | Colin V Beechey Simon T Ball K M Stuart Townsend Janet Jones |
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Institution: | (1) Mammalian Genetics Unit, Medical Research Council, Harwell, Didcot, Oxfordshire OX11 0RD, UK, GB |
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Abstract: | Mouse Chromosome (Chr) 7 distal to band F3 on the physical map is known to be subject to imprinting, maternal duplication
(MatDp) of the region leading to a late embryonic lethality, while paternal duplication (PatDp) causes death in utero before
11.5 dpc. Using a new mouse reciprocal translocation T(7;11)65H to produce MatDp for distal Chr 7, we have mapped the region
subject to imprinting more precisely to bands 7F4/F5 on the cytogenetic map. Fluorescence in situ hybridization (FISH) studies
on mitotic and meiotic chromosomes of a T65H heterozygote show that the imprinted gene Igf2 is located in the same region. This was confirmed by the finding that embryos with MatDp of bands 7F4/F5 did not express
Igf2. We suggest that other members of the imprinted domain containing Igf2, namely Mash2, H19, Ins2, and p57
K1P2
, are also located in 7F4/F5 and that some or all of these genes may be responsible for the two imprinting lethalities seen
with MatDp and PatDp for this region.
Received: 13 October 1996 / Accepted: 8 December 1996 |
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