Characterization of Rhizobial Isolates of Phaseolus vulgaris by Staircase Electrophoresis of Low-Molecular-Weight RNA |
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| Authors: | Encarna Velzquez Esperanza Martínez-Romero Dulce Nombre Rodríguez-Navarro Martha E Trujillo Antonio Daza Pedro F Mateos Eustoquio Martínez-Molina and Peter van Berkum |
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| Institution: | Encarna Velázquez, Esperanza Martínez-Romero, Dulce Nombre Rodríguez-Navarro, Martha E. Trujillo, Antonio Daza, Pedro F. Mateos, Eustoquio Martínez-Molina, and Peter van Berkum |
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| Abstract: | Low-molecular-weight (LMW) RNA molecules were analyzed to characterize rhizobial isolates that nodulate the common bean growing in Spain. Since LMW RNA profiles, determined by staircase electrophoresis, varied across the rhizobial species nodulating beans, we demonstrated that bean isolates recovered from Spanish soils presumptively could be characterized as Rhizobium etli, Rhizobium gallicum, Rhizobium giardinii, Rhizobium leguminosarum bv. viciae and bv. trifolii, and Sinorhizobium fredii. |
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