Calcium alginate immobilization of mammalian neurons: A potential tool to purify ligands of neuronal membrane proteins |
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| Authors: | Christian Tamponnet Sylvie Boisseau Chantal Poujeol Maurice Lievremont Michel Simonneau |
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| Affiliation: | (1) Laboratoire Interdisciplinaire de Recherches en Biologie Appliquée, Ecole Normale Supérieure de Cachan, 61 avenue du Président Wilson, F-94235 Cachan CEDEX, (France);(2) Laboratoire de Neurobiologie Cellulaire et Moleculaire, CNRS, F-91198 Gif-sur-Yvette, (France);(3) Present address: Life Support and Habitability Section, European Space Research and Technology Centre, European Space Agency, Postbus 299, 2200 AG Noordwijk, (The Netherlands) |
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| Abstract: | Summary We report here improved immobilization conditions which permitted (i) to immobilize mouse neuroblastoma cells in calcium alginate beads, (ii) to test the functions of using patch clamp techniques and (iii) to quantitatively analyze ligand interactions with voltage-dependent sodium channels in neurons immobilized inside alginate beads. These results qualify this immobilization technique as a biotechnological tool to isolate and/or purify ligands of neuronal membrane proteins. A part of these results was presented at the International Symposium “Physiology of immobilized Cells” at Wageningnen, The Netherlands, December 10–13, 1989 |
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