Aseptic ectomycorrhizal synthesis betweenAbies firma andCenococcum geophilum in artificial culture |
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| Authors: | Lu-Min Vaario Warwick M. Gill Megumi Tanaka Yuji Ide Kazuo Suzuki |
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| Affiliation: | (1) Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, 113-8657 Tokyo, Japan;(2) The University Forests, Faculty of Agriculture, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, 113-8657 Tokyo, Japan |
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| Abstract: | A simple in vitro system is described for the synthesis ofAbies firma-Cenococcum geophilum ectomycorrhizas. SterilizedA. firma seedlings on both MMN and FH media were inoculated with hyphal discs from actively growing margins ofC. geophilum colonies. Typical ectomycorrhizas formed on seedlings on FH medium after 3 mo of incubation. By light microscopy, the synthesized mycorrhizas were seen to possess a thin mantle from which emanated extraradicle hyphae and highly branched, rarely septate intracortical Hartig net mycelium, characteristic ectomycorrhizal features. This is the first report of aseptic ectomycorrhization ofA. firma seedlings byC. geophilum. This model system will facilitate detailed studies on ectomycorrhizal development ofAbies species. |
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| Keywords: | Abies firma Cenococcum geophilum ectomycorrhizas in vitro synthesis |
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