Crystallographic data for the DD-carboxypeptidase-endopeptidase of low penicillin sensitivity excreted by Streptomyces albus g.期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Crystallographic data for the DD-carboxypeptidase-endopeptidase of low penicillin sensitivity excreted by Streptomyces albus g.

Authors:	O Dideberg J M Frère J M Ghuysen

Affiliation:	Laboratoire de Cristallographie Institut de Physique B5 Université de Liège au Sart Tilman B-4000 Liège, Belgique;Service de Microbiologie, Faculté de Médecine Université de Liège au Sart Tilman Institut de Botanique B22 B-4000 Liège, Belgique

Abstract:	The dd-carboxypeptidase-endopeptidase of low penicillin sensitivity that is excreted by Streptomyces albus G has been crystallized from a polyethylene glycol (M_r 6000 to 7500) solution at pH 8.0. X-ray examination of the prismatic crystals shows that the space group is P2₁ with unit cell dimensions $a = 51.1 A ?, b = 49.7 A ?, c = 38.7 A ?, β = 100.6 °$ and one molecule in the asymmetric unit. A crystal suspension made in 50 mm-Tris · HCl buffer (pH 8.0) supplemented with 5 mm-MgCl₂ and 16% ( $w v$ ) polyethylene glycol exhibits enzyme activity on the substrate Ac₂-l-Lys-d-Ala-d-Ala.

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