| 多重RT-PCR用于临床检测三种胃肠炎病毒的研究 |
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| 引用本文： | 寇晓霞,吴清平,王大鹏,郭伟鹏,邓梅清. 多重RT-PCR用于临床检测三种胃肠炎病毒的研究[J]. 微生物学通报, 2007, 34(3): 0401-0405 |
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| 作者姓名： | 寇晓霞 吴清平 王大鹏 郭伟鹏 邓梅清 |
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| 作者单位： | 1. 广东省微生物研究所广东省菌种保藏与应用重点实验室,广州,510070;中国科学院武汉病毒研究所,武汉,430071;中国科学院研究生院,北京,100049
2. 广东省微生物研究所广东省菌种保藏与应用重点实验室,广州,510070 |
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| 摘 要： | 轮状病毒、诺瓦克病毒和星状病毒是引起病毒性胃肠炎的主要病原因子。研究采用JV12/JV13、P1/P2和Mon340/Mon348三对引物,建立了同时检测这3种病毒的多重RT-PCR技术,并应用于128份临床粪便样本的检测,检出轮状病毒62份(48.44%),诺瓦克病毒8份(6.25%),星状病毒11份(8.59%)。在灵敏度试验中,轮状病毒的检测灵敏度为5pg/mL、诺瓦克病毒和星状病毒的检测灵敏度均为50pg/mL。该研究所建立3种常见胃肠炎病毒的多重RT-PCR方法具有特异性强、灵敏度高的特点,可用于临床病原诊断和溯源。
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| 关 键 词： | 诺瓦克病毒 轮状病毒 星状病毒 多重RT-PCR |
| 文章编号： | 0253-2654（2007）03-0401-05 |
| 修稿时间： | 2006-10-18 |
Studies on Simultaneous Detection of Three Gastroenteritis Virus in Clinical Samples by Muptiplex RT-PCR |
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| KOU Xiao-Xi,WU Qing-Ping,WANG Da-Peng,GUO Wei-Peng and DENG Mei-Qing. Studies on Simultaneous Detection of Three Gastroenteritis Virus in Clinical Samples by Muptiplex RT-PCR[J]. Microbiology China, 2007, 34(3): 0401-0405 |
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| Authors: | KOU Xiao-Xi WU Qing-Ping WANG Da-Peng GUO Wei-Peng DENG Mei-Qing |
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| Affiliation: | 1. Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070;2. Wuhan Insthute of Virology, Chinese Academy of Science, Wuhan 430071 ; 3. Graduate University of Chinese Academy of Sciencee, Beijing 100049 |
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| Abstract: | Rotavirus,norwalk virus and astrovirus are the major causes of viral gastroenteritis.Primers JV12/JV13,P1/P2 and Mon340/Mon348 were used to develop a multiplex RT-PCR system to simultaneously detect norwalk virus,rotavirus and astrovirus in clinical fecal samples.In a total of 128 samples,62 was rotavirus,8 was norwalk virus and 11 was astrovirus.The detection limits was 5pg/mL for rotavirus,50pg/mL for norwalk virus and astrovirus respectively.The multiplex RT-PCR system can be used to detect norwalk virus,rotavirus and astrovirus for routine monitoring and risk assessment in diseases outbreak with high specificity and sensitivity. |
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| Keywords: | Norwalk virus Rotavirus Astrovirus Multiplex RT-PCR |
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