Characterization and stress-protective action of <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> extracellular peptide factors on propionic acid bacteria |
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| Authors: | L I Vorob’eva E A Rogozhin E Yu Khodzhaev R A Volodyashkin V A Samoilenko |
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| Institution: | 1.Faculty of Biology,Lomonosov Moscow State University,Moscow,Russia;2.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences,Moscow,Russia;3.Gause Institute of New Antibiotics,Moscow,Russia;4.Skryabin Institute of Biochemistry and Physiology of Microorganisms,Russian Academy of Sciences,Pushchino,Russia |
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| Abstract: | Protective effect of Saccharomyces cerevisiae exometabolites (RF1 and RF2) on seven strains of propionic acid bacteria under exposure to bile salts and acid stress was studied. RF2 had a stronger protective effect than RF1. Comparison of the analytical profiles of RF1 and RF2, separated by RP-HPLC, showed that nearly 80% of RF1 components were present in RF2. Separation of S. cerevisiae RF1 into individual components by means of semi-preparative RP-HPLC was used for structural characterization of the active component of the RF1 fraction. It was found to contain two pentapeptides (<1 kDa) with identical amino acid sequences (1Gly-Pro-Tre-Gly-Pro5), differing in one hexose residue. The RF2 separation method based on three-stage liquid chromatography at low and high pressure was developed, which involved analytical high-pressure gel chromatography. The use of the latter method made it possible to separate high-molecular components and perform enrichment of low-molecular peptides. Peptide origin of the RF2 active substances with molecular masses allegedly below 300 Da was confirmed. |
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