In Vitro Susceptibility of <Emphasis Type="Italic">C. albicans</Emphasis> and <Emphasis Type="Italic">C. neoformens</Emphasis> to Potential Metabolites from <Emphasis Type="Italic">Streptomycetes</Emphasis> |
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Authors: | K S Shobha R Onkarappa |
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Institution: | (1) Department of Microbiology, Sahyadri Science College (Autonomous), Kuvempu University, Shimoga, India |
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Abstract: | Forty two Streptomycetes isolates from soils of Kodachadri region in Western ghats were recovered by soil dilution technique. Cross streak method
was followed for primary screening of antifungal activity. Positive isolates were subjected to secondary screening by cold
extraction of fermentation broth in butanol solvent. Six isolates exhibited broad spectrum antifungal activity against all
the tested yeast pathogens like Candida albicans, Candida lipolytica,
Cryptococcus neoformens and Saccharomyces cerevisiae. One isolate showed excellent antifungal activity against all test organisms with maximum zone of inhibition 60 mm each incase
of C. neoformens and C. albicans. Partial characterization of antifungal metabolite by TLC resulted in a purple spot with an Rf value 0.50. The UV absorption spectra at 218 nm indicated possible chemical nature of the active metabolite as polyene group
and purity was assessed by analytical HPLC. |
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Keywords: | Antifungal activity Cross streak Secondary screening HPLC UV absorption |
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