<Emphasis Type="Italic">Paenibacillus polymyxa</Emphasis> JB05-01-1 and its perspectives for food conservation and medical applications |
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Authors: | Karim Naghmouchi Lyn Paterson Bob Forster Tim McAllister Sam Ohene-Adjei Djamel Drider Ron Teather John Baah |
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Institution: | (1) Lethbridge Research Center, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada, T1J 4B1;(2) Department of Research into Sanitary Risks and Biotechnology of Reproduction, UPSP 5301 DGER, Nantes-Atlantic National College of Veterinary Medicine, Food Science and Engineering (ONIRIS), ONIRIS La Chantrerie, BP 40706, 44307 Nantes Cedex 03, France;(3) Canadian Food Inspection Agency’s, Lethbridge, AB, Canada, T1J 3Z4; |
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Abstract: | The aim of this study was to isolate a novel bacterial strain with strong and broad spectrum antibacterial activity from a
livestock feed prebiotic supplement. A novel strain, termed Paenibacillus polymyxa JB05-01-1, was isolated using traditional microbiological methods and identified on the basis of its phenotypic and biochemical
properties as well as its 16S rRNA gene sequence. This strain was able to inhibit growth of gram-negative bacteria including
Escherichia coli RR1, Pseudomonas fluorescens R73, Pantoea agglomerans BC1, Butyrivibrio fibrisolvens OR85, and Fibrobacter succinogenes. The above antagonism against the aforementioned bacteria was attributed to production of an antimicrobial substance(s) termed
“JB05-01-1.” Its production was optimal during the stationary phase. JB05-01-1 has a molecular weight of 2.5 KDa, its mode
of action is bactericidal, and the divalent cations, Ca2+ and Mn2+, reduced its lethality. The antibacterial activity was heat-stable and was effective at a pH range of 2–9. Enzymes like trypsin,
α-chymotrypsin, and proteinase K have abolished the antibacterial activity of JB05-01-1 indicating a proteinaceous motif.
This type of naturally occurring bacteria and inhibitory substance(s) could represent an additional value in livestock feed
supplements. The natural presence of antibacterial activity indicates an opportunity to decrease the addition of antibiotics. |
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