<Emphasis Type="Italic">osa</Emphasis>-<Emphasis Type="Italic">MIR393</Emphasis>: a salinity- and alkaline stress-related microRNA gene |
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Authors: | Peng Gao Xi Bai Liang Yang Dekang Lv Xin Pan Yong Li Hua Cai Wei Ji Qin Chen Yanming Zhu |
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Institution: | (1) Plant Bioengineering Laboratory, Northeast Agricultural University, Harbin, 150030, China;(2) Lethbridge Research Centre, Agriculture and Agri-Food Canada, 5403-1 Ave, South P.O. Box 3000, Lethbridge, AB, T1J 4B1, Canada |
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Abstract: | Salinity and alkalinity are the two main environmental factors that limit rice production. Better understanding of the mechanisms
responsible for salinity and alkaline stress tolerance would allow researchers to modify rice to increase its resistance to
salinity and alkaline stress. MicroRNAs (miRNAs) are ~21-nucleotide RNAs that are ubiquitous regulators of gene expression
in eukaryotic organisms. Some miRNAs acts as an important endogenous regulator in plant responses to abiotic stressors. miR393
is a conservative miRNA family that occurs in a variety of different plants. The two members of the miR393 family found in
rice are named osa-MIR393 and osa-MIR393b. We found that the osa-MIR393 expression level changed under salinity and alkaline stress, whereas that of osa-MIR393b did not. Target genes of osa-MIR393 were predicted, and some of these putative targets are abiotic related genes. Furthermore, we generated transgenic rice and
Arabidopsis thaliana that over-expressed osa-MIR393, and the phenotype analysis showed that these transgenic plants were more sensitive to salt and alkali treatment compared
to wild-type plants. These results illustrate that over-expression of osa-MIR393 can negatively regulate rice salt-alkali stress tolerance. |
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