An Immunological Assay for Detection and Enumeration of Thermophilic Biomining Microorganisms |
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| Authors: | Ana M Amaro Kevin B Hallberg E Brje Lindstrm and Carlos A Jerez |
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| Institution: | Ana M. Amaro, Kevin B. Hallberg, E. Börje Lindström, and Carlos A. Jerez |
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| Abstract: | A specific, fast, and sensitive nonradioactive immunobinding assay for the detection and enumeration of the moderate thermophile Thiobacillus caldus and the thermophilic archaeon Sulfolobus acidocaldarius was developed. It employs enhanced chemiluminescence or peroxidase-conjugated immunoglobulins in a dot or slot blotting system and is very convenient for monitoring thermophilic bioleaching microorganisms in effluents from industrial bioleaching processes. |
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