Toxicity of Mercaptoethanol to Mutant Strains of the Yeast Pichia methanolica Growing on Different Carbon Sources |
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Authors: | Leonovich S A Serkova N N Rabinovich Ya M |
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Institution: | (1) Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 117071, Russia;(2) Institute for Genetics and Selection of Industrial Microorganisms, Moscow, 113545, Russia |
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Abstract: | Addition of -mercaptoethanol at a concentration of 2–3 mM to media containing methanol, glucose, or yeast extract caused a 50% inhibition of the growth of wild-type yeastPichia methanolica; mercaptoethanol at a concentration of 0.7 to 25 mM inhibited the growth of the mutant strain ecr1. The mutation mth1 of P. methanolica repressed its ability to consume methanol and was accompanied by the loss of alcohol oxidase (EC 1.1.3.13) activity. -Mercaptoethanol restored the ability of mth1 mutant cells to grow on methanol and stimulated their growth under derepression conditions. The growth effect of -mercaptoethanol during derepression was accompanied by partial restoration of alcohol oxidase activity. |
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