Purification of nucleic acids by hybridization to affinity tagged PNA probes |
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Authors: | Ørum H |
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Institution: | PNA Diagnostics A/S, R?nnegade 2, 6. Floor, 2100 Copenhagen ?, Denmark. |
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Abstract: | The use of affinity tagged PNA capture probes offers an efficient means for the purification of nucleic acids by hybridization. Two different approaches are described. A sequence specific method and a generic method. The sequence specific method requires sequence information on the target and synthesis of a dedicated PNA. It can be used to selectively purify the nucleic acid containing the target from non-related nucleic acids and other cellular components. The generic method uses a "universal" triplex forming PNA and requires no sequence information on the target. It can be used in the bulk purification of large nucleic acids. |
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