The two genes homologous to Arabidopsis FAE1 co-segregate with the two loci governing erucic acid content in Brassica napus |
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| Authors: | M Fourmann P Barret M Renard G Pelletier R Delourme D Brunel |
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| Institution: | (1) 1-INRA Station de Génétique et Amélioration des Plantes, Route de St Cyr, 78026 Versailles cedex, France Fax : (33) 01 30 83 33 08 E-mail: fourmann@versailles.inra.fr, FR;(2) 2-INRA Station d’Amélioration des Plantes, B.P. 29, 35650 Le Rheu, France, FR |
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| Abstract: | KCS (β-keto-acyl-CoA synthase) has been proposed as a candidate gene for explaining the erucic acid level in rapeseed. Degenerate
PCR primers corresponding to the FAE1 gene have been designed. Two B. napus genes BN-FAE1.1 and BN-FAE1.2, corresponding to the parental species B. rapa and B. oleracea FAE1 genes, were amplified. Polymorphism was revealed for these two genes by acrylamide electrophoresis of the amplification products.
These two genes could then be mapped and a co-segregation of these genes with the E1 and E2 loci controlling erucic acid content was found. Furthermore, mutations observed for one of these genes could explain part
of the low erucic trait of the three LEAR types used in this study.
Received: 3 November 1997 / Accepted: 25 November 1997 |
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| Keywords: | Fatty acid elongase Erucic acid Consensus primers Arabidopsis Brassica |
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