Demonstration of the genuine iso-12p character of the standard marker chromosome of testicular germ cell tumors and identification of further chromosome 12 aberrations by competitive in situ hybridization. |
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| Authors: | R F Suijkerbuijk A Y van de Veen J van Echten C H Buys B de Jong J W Oosterhuis D A Warburton J J Cassiman D Schonk and A Geurts van Kessel |
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| Institution: | Department of Human Genetics, University Hospital Nijmegen, The Netherlands. |
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| Abstract: | The recently developed competitive in situ hybridization (CISH) strategy was applied to the analysis of chromosome 12 aberrations in testicular germ cell tumors (TGCTs). DNAs from two rodent-human somatic cell hybrids, containing either a normal chromosome 12 or the p arm of chromosome 12 as their unique human material, were used as probes. Our results demonstrate a genuine iso-12p character of the standard marker chromosome in TGCTs. Moreover, variant markers were identified representing translocation products that also involve chromosome 12. |
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