|
|||||
|
|
Attempts to Stimulate Mitosis in Cultured Trophoblast Cells of Cattle Embryos |
|
| Authors: | Gustafsson H King WA Berglund B Picard L |
| Institution: | 110.Swedish Association for Livestock Breeding and Production, S-63184, Eskilstuna, Sweden ;210.Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences, Uppsala, Sweden ;310.Centre de Recherche en Reproduction Animate, Fac. Med. Vet. Uni-versité de Montréal, Sl-Hyacinthe, Québec, Canada ;410.Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden ; |
| Abstract: | Trophoblast from Day-14 bovine embryos was cultured in medium containing mitogens to determine if the mitotic index could be altered. Trophoblast from each of 15 embryos was cultured in minimum essential medium (Eagles) with 20 % fetal calf serum (control) or in this medium supplemented with pokeweed mitogen (1 %, v/v), phytohemagglutinin (1 %, v/v), concanavalin A (1 %, v/v) or thymidine (2 mg/ml). No mitogenic effect was observed due to any of the treatments. However, mitotic indexes were significantly lower when pokeweed (P < 0.05) or thymidine (P < 0.01) was added to the medium. A highly significant (P < 0.001) variation in mitotic index between embryos was observed. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |