Phylogenetic diversity of thermophilic carbohydrate degrading bacilli from Bulgarian hot springs |
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Authors: | Anna Derekova Rossica Mandeva Margarita Kambourova |
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Institution: | (1) Department of Extremophilic Bacteria, Institute of Microbiology, Bulgarian Academy of Sciences, acad. G. Bonchev str. 26, 1113 Sofia, Bulgaria |
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Abstract: | Phylogenetic diversity of culturable bacteria from genus Bacillus and related genera, isolated from 18 Bulgarian hot springs was investigated in association with their functional diversity.
Sixty-seven thermophilic and facultative thermophilic strains were isolated under aerobic conditions at 60°C. Sixty-six of
them belonged to eight species in four genera from Bacillus group: Anoxybacillus, Geobacillus, Brevibacillus and Bacillus. Representatives of the genus Anoxybacillus predominated. Based on phylogenetic analysis (<97% sequence similarity) four strains belonged to groups representing potentially
novel species. Producers of carbohydrases, degrading 12 from the tested 13 substrates were isolated. About half of the isolates
degraded amylose by exo- or endo-mechanism of action of their enzymes. The isolates degrading hemicellulose carbohydrates
like arabinan, arabinoxylan, β-glucan, galactan, galactomannan and xyloglucan were reached to. Some of the microorganisms
were able to uptake microbial polysaccharides like curdlan and gellan and their enzymes were between first reported thermostable
enzymes in their groups, like gellan lyase and curdlan lyase A relation between species affiliation and their functional activity
was observed—all A. gonensis strains were producer of amylolytic enzymes, most of Brevibacillus ruber strains were able to grow in a minimal medium with xanthan. |
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Keywords: | Carbohydrate degrading activity Continental hot springs Thermophilic bacilli Thermostable enzymes 16S rDNA analysis |
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