Rat sperm protamine. Isolation and sequence analysis |
| |
Authors: | H Ammer A Henschen |
| |
Institution: | Max-Planck-Institut für Biochemie, Martinsried bei München. |
| |
Abstract: | Rat protamine was extracted from S-(pyridylethylated) epididymal sperm cell nuclei with dilute hydrochloric acid. The final purification was achieved by reversed-phase high-performance liquid chromatography. The primary structure was determined by N-terminal sequencing of the total S-(pyridylethylated) protein, and of endoproteinase Lys-C- and thermolysin-derived fragments. Rat protamine consists of 50 amino-acid residues. It is a typical type 1 protamine and differs in two and ten positions from the corresponding mouse and rabbit protamine, respectively. Only 26 positions are invariant in all type 1 mammalian protamines. |
| |
Keywords: | |
|
|