A rapid and convenient screening technique for developmental pathway mutants of Saccharomyces cerevisiae |
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Authors: | J.Richard Dickinson Deborah Cole |
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Affiliation: | School or Pure and Applied Biology, University of Wales College of Cardiff, U.K. |
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Abstract: | A brief exposure to iodine vapour was used to screen for mutants of the yeast Saccharomyces cerevisiae affected in development. Besides obtaining a large number of asporogenous mutants, two novel mutations were identified that permitted germination of spores to occur in conditions (sporulation medium) in which the wild-type would not germinate. These two mutations were named gdr1 and gdr2 for germination derepressed. Both alter nutritional control of germination, but not the kinetics of germination in glucose-containing medium. |
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Keywords: | Saccharomyces cerevisiae Germination Sporulation Mutants |
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