The death-inducing signalling complex is recruited to lipid rafts in Fas-induced apoptosis |
| |
Authors: | Scheel-Toellner D Wang K Singh R Majeed S Raza K Curnow S J Salmon M Lord J M |
| |
Institution: | MRC Centre for Immune Regulation, Birmingham University Medical School, Birmingham B15 2TT, UK. |
| |
Abstract: | Membrane microdomains known as lipid rafts have been shown recently to be involved in Fas signalling and apoptosis in T and B cell lines. Here, we have investigated further the role of lipid rafts in Fas-induced apoptosis in non-transformed human CD4 T cells. We show that Fas-induced apoptosis in CD4 T cells was inhibited by the lipid raft disrupter methyl-beta-cyclodextrin. When lipid rafts were isolated from control and Fas ligand treated cells, we found that a small proportion of Fas was present in the raft fraction in untreated cells and that this was greatly increased upon Fas ligation. The other components of the Death Inducing Signalling Complex (DISC), FADD, and procaspase 8, were also present at higher levels in the raft fraction isolated from Fas ligand treated cells. We conclude that formation of the DISC occurs in lipid rafts and that these membrane microdomains are required for efficient Fas signalling and apoptosis. |
| |
Keywords: | DISC Fas FADD Apoptosis Lipid raft |
本文献已被 ScienceDirect PubMed 等数据库收录! |