A novel mass spectrometric procedure to rapidly determine the partial structure of heparin fragments |
| |
| Authors: | C J McNeal R D Macfarlane I Jardine |
| |
| Institution: | 1. KU Leuven Department of Materials Engineering (MTM), Biomaterials and Tissue Engineering Research Group, Kasteelpark Arenberg 44 – Box 2450, Leuven 3001, Belgium;2. KU Leuven Department of Chemistry, Laboratory for Organic & Microwave-Assisted Chemistry (LOMAC), Celestijnenlaan 200F – Box 2404, Leuven 3001, Belgium;3. KU Leuven Department of Biosystems, Laboratory of Gene Technology, Kasteelpark Arenberg 21 – Box 2462, Leuven 3001, Belgium;4. KU Leuven Department of Microbial and Molecular Systems, Centre of Microbial and Plant Genetics (CMPG), Kasteelpark Arenberg 20 – Box 2460, Leuven 3001, Belgium;5. Biomaterials Laboratory, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, 3-1-1, Tsushima, Kita-ku, Okayama 700-8530, Japan;6. Chemistry and Structure of Novel Materials, University of Siegen, Paul-Bonatz-Str. 9-11, Siegen 57076, Germany;7. Peoples’ Friendship University of Russia (RUDN University), Miklukho-Maklaya Street 6, Moscow 117198, Russian Federation |
| |
| Abstract: | The molecular weight and degree of sulfation has been obtained for di-, tetra- and hexasaccharide fragments of heparin obtained by enzymatic depolymerization of porcine mucosal heparin. The sodium salt form of the sulfated oligosaccharide is adsorbed onto an immobilized cationic surfactant film which is inserted directly into the mass spectrometer. Analyses are routinely obtained on 25-50 microgram samples in less than an hour. This approach provides rapid confirmatory structural information that is complementary to existing methodologies. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
