Intrinsic and extrinsic fluorescence probes of subunit interactions in ovine lutropin.

The fluorescence of 1,8-anilinonaphthalene sulfonate (ANS) was enhanced in the presence of ovine lutropin (oLH). Fluorescence titration curves were sigmoidal with 50% saturation between 200 and 500 μm. Exclusion chromatography experiments indicated that the hormone self-associates to form dimers in the presence of excess ANS. By contrast, the isolated a and β subunits of oLH caused a much smaller enhancement of the fluorescence of ANS and did not self-associate in its presence. Dissociation of the intact hormone into its subunits was accompanied by 1) a loss in the ability to enhance ANS fluorescence, 2) the appearance of a negative differential absorption spectrum whose magnitude indicated the increased solvent-exposure of at least two tyrosines, and 3) a loss in conformational rigidity as evidenced by a decrease in polarization (P) of tyrosyl fluorescence from ~0.17 to ~0.13. Similar rates of dissociation were obtained by all three measurements and the first order rate constant at pH 3.6 and 37 °C under conditions of low ionic strength was k = 0.18 min^?1; at high ionic strength, e.g., 0.5 m KC1, dissociation was incomplete even after prolonged incubation. Acid-dissociated subunits recombined readily in 0.5 m acetate buffer, pH 5.3, and the recovery of the intrinsic absorption and fluorescence properties as well as the ability to enhance ANS fluorescence ranged between 70 and 90%. Titration of the isolated α and β subunits with acid or GdmCl had little or no effect on P, suggesting that residual secondary or tertiary structure is either absent, very stable, or its disruption does not alter the rigidity of the tyrosyl environment. The relatively high P for oLH-β (0.17) suggests a conformation which is rigid compared with oLH-α (0.13). P for both subunits decreased smoothly with increasing temperature between 20 and 70 °C. By contrast, oLH exhibited a thermal transition near 50 °C characterized by a drop in P from a value near that of β to a value near that of a as the subunits dissociated. Because α has more tyrosines with a higher average quantum yield, its fluorescence would be expected to dominate that of the hormone or of an equimolar mixture of subunits. Thus, most of the conformation changes which accompany dissociation and recombination appear to occur in the α subunit.