A radiation-reduced hybrid cell line containing 5 Mb/17 cM of human DNA from 9q34. |
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| Authors: | E P Henske L Ozelius M A Anderson D J Kwiatkowski |
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| Institution: | Division of Experimental Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115. |
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| Abstract: | Disease gene loci for tuberous sclerosis (TSC1), idiopathic torsion dystonia (DYT1), and nail-patella syndrome (NPS1) have been mapped by genetic linkage analysis to human chromosome 9q band 34. To create a resource for physical mapping and manipulation of this region of the genome, we have created a radiation-reduced hybrid cell line containing DNA from human 9q34 as its only human component. This cell line, E6B, has been characterized by Southern blot and PCR analysis using a panel of 9q markers and fluorescent in situ hybridization. We estimate that it contains 5 Mb of human DNA, equal to 17 cM of genetic distance, extending from AK1 to ABO on 9q34. |
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