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Expression of novel rice gibberellin 2-oxidase gene is under homeostatic regulation by biologically active gibberellins
Authors:Miho?Sakai  Tomoaki?Sakamoto  Tamio?Saito  Makoto?Matsuoka  Hiroshi?Tanaka  Email author" target="_blank">Masatomo?KobayashiEmail author
Institution:(1) , National Institute of Agrobiological Sciences, Tsukuba, Japan,;(2) Field Production Science Center, The University of Tokyo, Nishi-Tokyo, Japan,;(3) , RIKEN Tsukuba Institute, 3–1–1 Koyadai, Tsukuba, Ibaraki 305–0074, Japan,;(4) BioScience Center, Nagoya University, Nagoya, Japan,;(5) School of Engineering, Nippon Bunri University, Oita, Japan,
Abstract:We have cloned two genes for gibberellin (GA) 2-oxidase from rice (Oryza sativa L.). Expression of OsGA2ox2 was not observed. The other gene, OsGA2ox3, was expressed in every tissue examined and was enhanced by the application of biologically active GA. Recombinant OsGA2ox3 protein catalyzed the metabolism of GA1 to GA8 and GA20 to GA29-catabolite. These results indicate that OsGA2ox3 is involved in the homeostatic regulation of the endogenous level of biologically active GA in rice. Electronic Publication
Keywords:Elongation GA 2-oxidase Oryza sativa
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