DU-145 prostate carcinoma cells that selectively transmigrate narrow obstacles express elevated levels of Cx43 |
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Authors: | Katarzyna Szpak Ewa Wybieralska Ewa Niedzia?kowska Monika Rak Iga Bechyne Marta Michalik Zbigniew Madeja Jaros?aw Czy? |
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Institution: | (1) Division of Cardiology, Department of Internal Medicine, Geneva University Hospitals, University of Geneva, Geneva, Switzerland;(2) Division of Angiology and Hemostasis, Department of Internal Medicine, Geneva University Hospitals, University of Geneva, Geneva, Switzerland;(3) Service and Central Laboratory of Hematology, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Geneva, Switzerland;(4) Foundation for Medical Research, Division of Cardiology, Geneva University Hospitals, 64 Av. De la Roseraie, 1211 Geneva 4, Switzerland; |
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Abstract: | The formation of aqueous intercellular channels mediating gap junctional intercellular coupling (GJIC) is a canonical function
of connexins (Cx). In contrast, mechanisms of GJIC-independent involvement of connexins in cancer formation and metastasis
remain a matter of debate. Because of the role of Cx43 in the determination of carcinoma cell invasive potential, we addressed
the problem of the possible Cx43 involvement in early prostate cancer invasion. For this purpose, we analysed Cx43-positive
DU-145 cell subsets established from the progenies of the cells most readily transmigrating microporous membranes. These progenies
displayed motile activity similar to the control DU-145 cells but were characterized by elevated Cx43 expression levels and
GJIC intensity. Thus, apparent links exist between Cx43 expression and transmigration potential of DU-145 cells. Moreover,
Cx43 expression profiles in the analysed DU-145 subsets were not affected by intercellular contacts and chemical inhibition
of GJIC during the transmigration. Our observations indicate that neither cell motility nor GJIC determines the transmigration
efficiency of DU-145 cells. However, we postulate that selective transmigration of prostate cancer cells expressing elevated
levels of Cx43 expression may be crucial for the “leading front” formation during cancer invasion. |
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