Galactose epimeraseless (GalE) mutant G30 of Salmonella typhimurium is a good potential live oral vaccine carrier for fimbrial antigens |
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Authors: | Gordon Stevenson Paul A. Manning |
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Affiliation: | Department of Botany and Microbiology, Arizona State University, Tempe, AZ 85287, U.S.A. |
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Abstract: | Abstract Extracts of the transformable cyanobacterial strain Anacystis nidulans R2 were analyzed for the presence of restriction endonuclease. One enzyme, Ani I, was found and determined to be sequence-specific on the basis of its ability to cleave several Bacillus plasmids at a limited number of sites. The activity of this enzyme is significantly reduced in extracts prepared from cell cultures grown at 38°C. |
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Keywords: | Restriction endonuclease Anacystis nidulans transformation |
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