Cyclic mononucleotides modulate potassium and calcium flux responses to H2O2 in Arabidopsis roots |
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| Authors: | Natalia Maria Ordoñ ez,Claudius Marondedze,Ludivine Thomas,Stefania Pasqualini,Lana Shabala,Sergey Shabala,Chris Gehring |
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| Affiliation: | 1. Biological and Environmental Sciences & Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia;2. Bioscience Core Facility, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia;3. Department of Applied Biology, University of Perugia, Borgo XX Giugno, 74, 06121 Perugia, Italy;4. School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, Tasmania 7001, Australia |
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| Abstract: | Cyclic mononucleotides are messengers in plant stress responses. Here we show that hydrogen peroxide (H2O2) induces rapid net K+-efflux and Ca2+-influx in Arabidopsis roots. Pre-treatment with either 10 μM cAMP or cGMP for 1 or 24 h does significantly reduce net K+-leakage and Ca2+-influx, and in the case of the K+-fluxes, the cell permeant cyclic mononucleotides are more effective. We also examined the effect of 10 μM of the cell permeant 8-Br-cGMP on the Arabidopsis microsomal proteome and noted a specific increase in proteins with a role in stress responses and ion transport, suggesting that cGMP is sufficient to directly and/or indirectly induce complex adaptive changes to cellular stresses induced by H2O2. |
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| Keywords: | Plant stress Hydrogen peroxide Cyclic mononucleotide cAMP cGMP Ion flux Proteomic Arabidopsis thaliana |
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