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Erythroid differentiation is augmented in Reelin-deficient K562 cells and homozygous reeler mice |
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| Authors: | Hui-Chun Chu Hsing-Ying Lee Yen-Shu Huang Wei-Lien Tseng Ching-Ju Yen Ju-Chien Cheng Ching-Ping Tseng |
| Institution: | 1. Graduate Institute of Biomedical Science, College of Medicine, Chang Gung University, Kwei-Shan, Taoyuan 333, Taiwan, ROC;2. Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Kwei-Shan, Taoyuan 333, Taiwan, ROC;3. Department of Medical Laboratory Sciences and Biotechnology, China Medical University, Taichung 404, Taiwan, ROC;4. Molecular Medicine Research Center, Chang Gung University, Kwei-Shan, Taoyuan 333, Taiwan, ROC |
| Abstract: | Reelin is an extracellular glycoprotein that is highly conserved in mammals. In addition to its expression in the nervous system, Reelin is present in erythroid cells but its function there is unknown. We report in this study that Reelin is up-regulated during erythroid differentiation of human erythroleukemic K562 cells and is expressed in the erythroid progenitors of murine bone marrow. Reelin deficiency promotes erythroid differentiation of K562 cells and augments erythroid production in murine bone marrow. In accordance with these findings, Reelin deficiency attenuates AKT phosphorylation of the Ter119+CD71+ erythroid progenitors and alters the cell number and frequency of the progenitors at different erythroid differentiation stages. A regulatory role of Reelin in erythroid differentiation is thus defined. |
| Keywords: | EPO erythropoietin FBS fetal bovine serum Hct hematocrit Hb hemoglobin HRP horseradish peroxidase MCH mean corpuscular hemoglobin MCHC mean corpuscular hemoglobin concentration MCV mean corpuscular volume NaB sodium butyrate NFAT nuclear factor of activated T cells PAGE polyacrylamide gel electrophoresis PBS phosphate-buffered saline PVDF polyvinylidene fluoride RDW red blood cell distribution width RPMI Roswell Park Memorial Institute SDS sodium dodecyl sulfate TMB 3 3&prime 5 5&prime -tetramethylbenzidine |
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