Morphofunctional characterization of decellularized vena cava as tissue engineering scaffolds |
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Authors: | Matheus Bertanha,Andrei Moroz,Rodrigo G. Jaldin,Regina A.M. Silva,Jaqueline C. Rinaldi,Má rjorie A. Golim,Sé rgio L. Felisbino,Maria A.C. Domingues,Marcone L. Sobreira,Patricia P. Reis,Elenice Deffune |
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Affiliation: | 1. Department of Surgery and Orthopedics, Botucatu Medical School, São Paulo State University (UNESP), Vascular Laboratory, Avenue Prof. Montenegro, S/N. Rubião Júnior, Botucatu 18618-970, SP, Brazil;2. Blood Transfusion Center, Cell Engineering Laboratory, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil;3. Department of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, SP, Brazil;4. Department of Morphology, Extracellular Matrix Laboratory, Botucatu Biosciences Institute, São Paulo State University (UNESP), Botucatu, SP, Brazil;5. Blood Transfusion Center, Flow Cytometry Laboratory, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil;6. Department of Pathology, Immunohistochemistry Laboratory, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil;g Department of Urology, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil |
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Abstract: | Clinical experience for peripheral arterial disease treatment shows poor results when synthetic grafts are used to approach infrapopliteal arterial segments. However, tissue engineering may be an option to yield surrogate biocompatible neovessels. Thus, biological decellularized scaffolds could provide natural tissue architecture to use in tissue engineering, when the absence of ideal autologous veins reduces surgical options. The goal of this study was to evaluate different chemical induced decellularization protocols of the inferior vena cava of rabbits. They were decellularized with Triton X100 (TX100), sodium dodecyl sulfate (SDS) or sodium deoxycholate (DS). Afterwards, we assessed the remaining extracellular matrix (ECM) integrity, residual toxicity and the biomechanical resistance of the scaffolds. Our results showed that TX100 was not effective to remove the cells, while protocols using SDS 1% for 2 h and DS 2% for 1 h, efficiently removed the cells and were better characterized. These scaffolds preserved the original organization of ECM. In addition, the residual toxicity assessment did not reveal statistically significant changes while decellularized scaffolds retained the equivalent biomechanical properties when compared with the control. Our results concluded that protocols using SDS and DS were effective at obtaining decellularized scaffolds, which may be useful for blood vessel tissue engineering. |
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Keywords: | Peripheral arterial disease Blood vessels Tissue engineering Biomechanics Extracellular matrix |
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