RBM10 regulates alternative splicing |
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| Authors: | Akira Inoue Naoki Yamamoto Masatsugu Kimura Koji Nishio Hideo Yamane Koichi Nakajima |
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| Institution: | 1. Department of Immunology, Osaka City University Graduate School of Medicine, Japan;2. Department of Otolaryngology, Osaka City University Graduate School of Medicine, Japan;3. Department of Psychiatry and Behavioral Sciences, Tokyo Medical and Dental University Graduate School, Japan;4. Department of Radioisotope Center, Osaka City University Graduate School of Medicine, Japan;5. Department of Anatomy and Cell Biology, Graduate School of Medicine, Nagoya University, Japan |
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| Abstract: | RBM10, originally called S1-1, is a nuclear RNA-binding protein with domains characteristic of RNA processing proteins. It has been reported that RBM10 constitutes spliceosome complexes and that RBM5, a close homologue of RBM10, regulates alternative splicing of apoptosis-related genes, Fas and cFLIP. In this study, we examined whether RBM10 has a regulatory function in splicing similar to RBM5, and determined that it indeed regulates alternative splicing of Fas and Bcl-x genes. RBM10 promotes exon skipping of Fas pre-mRNA as well as selection of an internal 5′-splice site in Bcl-x pre-mRNA. We propose a consensus RBM10-binding sequence at 5′-splice sites of target exons and a mechanistic model of RBM10 action in the alternative splicing. |
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| Keywords: | S1-1 RBM10 RBM5 Alternative splicing Fas Bcl-x |
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