Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp. PCC 6803 |
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| Authors: | Ancy Joseph Shimpei Aikawa Kengo Sasaki Hiroshi Teramura Tomohisa Hasunuma Fumio Matsuda Takashi Osanai Masami Yokota Hirai Akihiko Kondo |
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| Institution: | 1. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan;2. Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan;3. Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, 3-5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan;4. RIKEN Biomass Engineering Program, 1-7-22, Suehirocho, Tsurumi, Yokohama 230-0045, Japan;5. Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University,1-5 Yamadaoka, Suita, Osaka 565-0871, Japan;6. PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan;g RIKEN Center for Sustainable Resource Science, 1-7-22, Suehirocho, Tsurumi, Yokohama 230-0045, Japan |
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| Abstract: | Rre37 (sll1330) in a cyanobacterium Synechocystis sp. PCC 6803 acts as a regulatory protein for sugar catabolic genes during nitrogen starvation. Low glycogen accumulation in Δrre37 was due to low expression of glycogen anabolic genes. In addition to low 2-oxoglutarate accumulation, normal upregulated expression of genes encoding glutamate synthases (gltD and gltB) as well as accumulation of metabolites in glycolysis (fructose-6-phosphate, fructose-1,6-bisphosphate, and glyceraldehyde-3-phosphate) and tricarboxylic acid (TCA) cycle (oxaloacetate, fumarate, succinate, and aconitate) were abolished by rre37 knockout. Rre37 regulates 2-oxoglutarate accumulation, glycogen accumulation through expression of glycogen anabolic genes, and TCA cycle metabolites accumulation. |
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| Keywords: | GS glutamine synthetase GOGAT glutamate synthase HPLC high performance liquid chromatography LC/QqQ-MS liquid chromatography/triple quadrupole mass spectrometry OD optical density TCA tricarboxylic acid 2-OG 2-oxoglutarate |
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