miR-202 suppresses cell proliferation in human hepatocellular carcinoma by downregulating LRP6 post-transcriptionally |
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| Authors: | Yi Zhang Dayong Zheng Yan Xiong Chengbiao Xue Gen Chen Bibo Yan Qifa Ye |
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| Affiliation: | 1. Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan, Hubei 430071, PR China;2. The 3rd Xiangya Hospital of Central South University, Research Center of National Health Ministry on Transplantation Medicine Engineering and Technology, Changsha 410013, PR China;3. Department of Oncology Nanfang Hosptial, Southern Medical University, Guangzhou 510515, PR China |
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| Abstract: | MicroRNAs have emerged as important regulators of carcinogenesis. In the current study, we observed that microRNA-202 (miR-202) is downregulated in hepatocellular carcinoma (HCC) cells and tissues, indicating a significant correlation between miR-202 expression and HCC progression. Overexpression of miR-202 in HCC cells suppressed cell proliferation and tumorigenicity, while downregulation of miR-202 enhanced the cells’ proliferative capacity. Furthermore, we identified low-density lipoprotein receptor-related protein 6 (LRP6) as a direct target of miR-202. miR-202 suppresses the expression of LRP6 by binding to the 3′-untranslated region (UTR) of its mRNA. Finally, we found that silencing the expression of LRP6 is the essential biological function of miR-202 during HCC cell proliferation. Collectively, our findings reveal that miR-202 is a potential tumor suppressive miRNA that participates in carcinogenesis of human HCC by suppressing LRP6 expression. |
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| Keywords: | miR, MicroRNAs HCC, hepatocellular carcinoma LRP, lipoprotein receptor-related protein 6 UTR, untranslated region LDL, low-density lipoprotein ATCC, American Type Culture Collection GAPDH, glyceraldehyde phosphate dehydrogenase PVDF, polyvinylidene fluoride |
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