Malignant cells derived from 3T3 fibroblast feeder layer in cell culture for nasopharyngeal carcinoma |
| |
| Authors: | Fenggang Yu Wen-son Hsieh Fredrik Petersson Henry Yang Yingying Li Chunwei Li Soon Wah Low Jing Liu Yan Yan De-Yun Wang Kwok Seng Loh |
| |
| Affiliation: | 1. Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore;2. Cancer Science Institute of Singapore, National University of Singapore, Singapore;3. Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore;4. Head & Neck Tumor group, National Cancer Institute of Singapore, Head & Neck Surgery, NUHS, Singapore |
| |
| Abstract: | In this study, we discovered a subpopulation of 3T3 feeder cells were malignantly transformed by nasopharyngeal carcinoma (NPC) tumor cells during co-culture. The transformed 3T3 cells acquired an accelerated growth rate, displayed loosely attached multilayer growth in vitro and highly tumorigenic in vivo. Most strikingly, instead of forming sarcomas, they developed into carcinoma-like tumors somewhat resembling the original NPC. We further demonstrated the transformation is not a single isolated event, rather a common reproducible, cell contact dispensable phenomena among NPC tumor cells. However, NPC tumor cells alone were not sufficient to confer the transformed characteristics onto normal human cells. |
| |
| Keywords: | Nasopharyngeal carcinoma Feeder layer 3T3 cells Transform Cell contact |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
