Galvanic microparticles increase migration of human dermal fibroblasts in a wound-healing model via reactive oxygen species pathway |
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Authors: | Nina Tandon Elisa Cimetta Aranzazu Villasante Nicolette Kupferstein Michael D. Southall Ali Fassih Junxia Xie Ying Sun Gordana Vunjak-Novakovic |
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Affiliation: | 1. Columbia University, Department of Biomedical Engineering, 622 West 168th Street, MC 104B, New York 10027, NY, USA;2. The Cooper Union for the Advancement of Science and Art, Department of Electrical Engineering, 41 Cooper Square, New York 10003, NY, USA;3. Johnson and Johnson Skin Research Center, Johnson & Johnson Consumer and Personal Products Worldwide, Division of Johnson & Johnson Consumer Companies, Inc., Skillman 08558, NJ, USA |
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Abstract: | Electrical signals have been implied in many biological mechanisms, including wound healing, which has been associated with transient electrical currents not present in intact skin. One method to generate electrical signals similar to those naturally occurring in wounds is by supplementation of galvanic particles dispersed in a cream or gel. We constructed a three-layered model of skin consisting of human dermal fibroblasts in hydrogel (mimic of dermis), a hydrogel barrier layer (mimic of epidermis) and galvanic microparticles in hydrogel (mimic of a cream containing galvanic particles applied to skin). Using this model, we investigated the effects of the properties and amounts of Cu/Zn galvanic particles on adult human dermal fibroblasts in terms of the speed of wound closing and gene expression. The collected data suggest that the effects on wound closing are due to the ROS-mediated enhancement of fibroblast migration, which is in turn mediated by the BMP/SMAD signaling pathway. These results imply that topical low-grade electric currents via microparticles could enhance wound healing. |
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Keywords: | Galvanic particles Reactive oxygen species Fibroblasts Wound healing |
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