Proteasome involvement in a complex cascade mediating SigT degradation during differentiation of Streptomyces coelicolor |
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| Authors: | Xu-Ming Mao Ni-Ni Ren Ning Sun Feng Wang Ri-Cheng Zhou Yi Tang Yong-Quan Li |
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| Affiliation: | 1. Zhejiang University, College of Life Sciences, Hangzhou 310058, China;2. Key Laboratory of Microbial Biochemistry and Metabolism Engineering of Zhejiang Province, Hangzhou 310058, China;3. Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA |
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| Abstract: | In Streptomyces coelicolor, the ECF sigma factor SigT negatively regulates cell differentiation, and is degraded by ClpP protease in a dual positive feedback manner. Here we further report that the proteasome is required for degradation of SigT, but not for degradation of its anti-sigma factor RstA, and RstA can protect SigT from degradation independent of the proteasome. Meanwhile, deletion of the proteasome showed reduced production of secondary metabolites, and the fermentation medium from wild type could promote SigT degradation. Furthermore, overexpression of redD or actII-orf4 in the proteasome-deficiency mutant resulted in SigT degradation and over-production of both undecylprodigiosin and actinorhodin. Therefore the proteasome is required for SigT degradation by affecting the production of secondary metabolites during cell differentiation. |
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| Keywords: | Proteasome ClpP protease Sigma factor Protein degradation Cell differentiation Streptomyces |
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