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A simple method for the determination of reduction potentials in heme proteins |
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| Authors: | Igor Efimov Gary Parkin Elizabeth S. Millett Jennifer Glenday Cheuk K. Chan Holly Weedon Harpreet Randhawa Jaswir Basran Emma L. Raven |
| Affiliation: | 1. Department of Chemistry, Henry Wellcome Building, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom;2. Department of Biochemistry, Henry Wellcome Building, University of Leicester, University Road, Leicester LE1 9HN, United Kingdom |
| Abstract: | We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (Em = −115 mV ± 2 mV, pH 7.0) and human CLOCK (Em = −111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins. |
| Keywords: | Heme Heme protein Reduction potential Redox |
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