2,3-Dihydroxy-quinoxaline induces ATPase activity of Herpes Simplex Virus thymidine kinase |
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| Authors: | Alexey A Zeifman Fedor N Novikov Victor S Stroylov Oleg V Stroganov Ghermes G Chilov Alexander Y Skoblov Anatoly I Miroshnikov Yuri S Skoblov |
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| Institution: | 1. N.D. Zelinsky Institute Of Organic Chemistry (ZIOC RAS), Leninsky prospekt, 47, 119991 Moscow, Russia;2. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia |
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| Abstract: | 2,3-Dihydroxy-quinoxaline, a small molecule that promotes ATPase catalytic activity of Herpes Simplex Virus thymidine kinase (HSV-TK), was identified by virtual screening. This compound competitively inhibited HSV-TK catalyzed phosphorylation of acyclovir with Ki = 250 μM (95% CI: 106–405 μM) and dose-dependently increased the rate of the ATP hydrolysis with KM = 112 μM (95% CI: 28–195 μM). The kinetic scheme consistent with this experimental data is proposed. |
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| Keywords: | DHQ 2 3-dihydroxy-quinoxaline HSV-TK Herpes Simplex Virus thymidine kinase TLC thin layer chromatography |
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