Functional analysis of the C-terminal region of the vacuolar cadmium-transporting rice OsHMA3 |
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| Authors: | Saori Kumagai Tatsuya Suzuki Kouichi Tezuka Namiko Satoh-Nagasawa Hidekazu Takahashi Kenji Sakurai Akio Watanabe Tatsuhito Fujimura Hiromori Akagi |
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| Institution: | 1. Graduate School of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan;2. Laboratory of Plant Genetics and Breeding, Faculty of Bioresource Sciences, Akita Prefectural University, Kaidoubata-Nishi 241-438, Shimoshinjyo-Nakano, Akita 010-0195, Japan;3. Faculty of Life and Environmental Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, 305-8572 Ibaraki, Japan |
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| Abstract: | Rice OsHMA3 is a vacuolar cadmium (Cd) transporter belonging to the P1B-ATPase family and has a long (273aa) C-terminal region. We analyzed the function of the region related to Cd using the transgenic Arabidopsis Col-0 ecotype, which is sensitive to Cd. The OsHMA3 variant containing a truncated (58aa) C-terminal region did not confer Cd tolerance, whereas an OsHMA3 variant containing a longer truncated (105aa) C-terminal region conferred Cd tolerance to transgenic Arabidopsis. We conclude that the C-terminal region, particularly the region containing the first 105aa, has an important role in OsHMA3 activity. |
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| Keywords: | P1B-ATPase (HMA) Cadmium Vacuolar sequestration C-terminal domain Rice OsHMA3 |
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