Attenuation of hydrogen peroxide-mediated oxidative stress by Brassica juncea annexin-3 counteracts thiol-specific antioxidant (TSA1) deficiency in Saccharomyces cerevisiae |
| |
| Authors: | Ahan Dalal Abhaypratap Vishwakarma Naveen Kumar Singh Triveni Gudla Mrinal Kanti Bhattacharyya Kollipara Padmasree Andrea Viehhauser Karl-Josef Dietz Pulugurtha Bharadwaja Kirti |
| |
| Affiliation: | 1. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India;2. Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India;3. Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad, India;4. Department of Plant Biochemistry and Physiology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany |
| |
| Abstract: | Brassica juncea annexin-3 (BjAnn3) was functionally characterized for its ability to modulate H2O2-mediated oxidative stress in Saccharomyces cerevisiae. BjAnn3 showed a significant protective role in cellular-defense against oxidative stress and partially alleviated inhibition of mitochondrial respiration in presence of exogenously applied H2O2. Heterologous expression of BjAnn3 protected membranes from oxidative stress-mediated damage and positively regulated antioxidant gene expression for ROS detoxification. We conclude that, BjAnn3 partially counteracts the effects of thioredoxin peroxidase 1 (TSA1) deficiency and aids in cellular-protection across kingdoms. Despite partial compensation of TSA1 by BjAnn3 in cell-viability tests, the over-complementation in ROS-related features suggests the existence of both redundant (e.g. ROS detoxification) and distinct features (e.g. membrane protection versus proximity-based redox regulator) of both proteins. |
| |
| Keywords: | BjAnn3, Brassica juncea annexin-3 TSA1, thioredoxin peroxidase 1 ROS, reactive oxygen species SOD1, superoxide dismutase 1 SOD2, superoxide dismutase 2 GPX2, glutathione peroxidase 2 TSA2, thioredoxin peroxidase 2 |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
