MiR-489 regulates chemoresistance in breast cancer via epithelial mesenchymal transition pathway |
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| Authors: | Li Jiang Dongxu He Dantong Yang Zhen Chen Qiongxi Pan Aiqin Mao Yanfei Cai Xiyuan Li Hui Xing Mei Shi Yun Chen Iain C Bruce Teng Wang Linfang Jin Xiaowei Qi Dong Hua Jian Jin Xin Ma |
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| Institution: | 1. School of Pharmaceutical Sciences, Jiangnan University, 1800 Lihu Rd, Wuxi, Jiangsu 214122, China;2. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China;3. Affiliated Hospital, Jiangnan University, Wuxi, China |
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| Abstract: | To investigate the role of microRNAs in the development of chemoresistance and related epithelial–mesenchymal transition (EMT), we examined the effect of miR-489 in adriamycin (ADM)-resistant human breast cancer cells (MCF-7/ADM). MiR-489 was significantly suppressed in MCF-7/ADM cells compared with chemosensitive parental control MCF-7/WT cells. Forced-expression of miR-489 reversed chemoresistance. Furthermore, Smad3 was identified as the target of miR-489 and is highly expressed in MCF-7/ADM cells. Forced expression of miR-489 both inhibited Smad3 expression and Smad3 related EMT properties. Finally, the interactions between Smad3, miR-489 and EMT were confirmed in chemoresistant tumor xenografts and clinical samples, indicating their potential implication for treatment of chemoresistance. |
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| Keywords: | microRNAs Chemoresistance Breast cancer Epithelial mesenchymal transition |
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