Hepatitis C virus core protein regulates NANOG expression via the stat3 pathway |
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Authors: | Jia-Jia Zhou Ru-Fu Chen Xiao-Geng Deng Yu Zhou Xiao Ye Min Yu Jing Tang Xiao-Yu He Di Cheng Bing Zeng Quan-bo Zhou Zhi-hua Li |
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Affiliation: | 1. Department of Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China;2. Department of Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China;3. Laboratory of Biomechanics and Physiology, Guangdong Provincial Institute of Sports Science, Guangzhou, Guangdong Province, China |
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Abstract: | HCV Core plays a role in the development of hepatocellular carcinoma. Aberrant expression of NANOG has been observed in many types of human malignancies. However, relationship between Core and NANOG has not been clarified. In this study, we found that Core is capable of up-regulating NANOG expression. Core-induced NANOG expression was accompanied by enforced expression of phosphorylated stat3 protein and was attenuated by inhibition of stat3 phosphorylation. ChIP showed that phosphorylated stat3 directly binds to the NANOG promoter. Core-induced NANOG expression resulted in enhanced cell growth and cell cycle progression. Knockdown of NANOG blocked the cell cycle at the G0/G1 phases and inhibited the cyclin D1 expression. Our findings provide a new insight into the mechanism of hepatocarcinogenesis by HCV infection. |
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Keywords: | HCV, hepatitis C virus HCC, hepatocellular carcinoma ChIP, chromatin immunoprecipitation |
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