Heterogeneity of glycolytic oscillatory behaviour in individual yeast cells |
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Authors: | Anna-Karin Gustavsson David D van Niekerk Caroline B Adiels Mattias Goksör Jacky L Snoep |
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Institution: | 1. Department of Physics, University of Gothenburg, SE-41296 Gothenburg, Sweden;2. Department of Biochemistry, Stellenbosch University, SA-7602 Matieland, South Africa;3. Molecular Cell Physiology, Vrije Universiteit, NL-1081 Amsterdam, The Netherlands;4. MIB, University of Manchester, GB-M13-9PL Manchester, UK |
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Abstract: | There are many examples of oscillations in biological systems and one of the most investigated is glycolytic oscillations in yeast. These oscillations have been studied since the 1950s in dense, synchronized populations and in cell-free extracts, but it has for long been unknown whether a high cell density is a requirement for oscillations to be induced, or if individual cells can oscillate also in isolation without synchronization. Here we present an experimental method and a detailed kinetic model for studying glycolytic oscillations in individual, isolated yeast cells and compare them to previously reported studies of single-cell oscillations. The importance of single-cell studies of this phenomenon and relevant future research questions are also discussed. |
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Keywords: | Glycolysis Limit-cycle oscillation Cell heterogeneity Detailed kinetic model Microfluidics Single-cell analysis |
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