A mammary repopulating cell population characterized in mammary anlagen reveals essential mammary stroma for morphogenesis |
| |
Authors: | Jiazhe Song Kai Xue Ji She Fangrong Ding Song Li Rulan Shangguan Yunping Dai Liying Du Ning Li |
| |
Affiliation: | 1. State Key Laboratories for AgroBiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China;2. College of Basic Medical Sciences, Dalian Medical University, Dalian, PR China;3. College of Life Sciences, Peking University, Beijing, PR China;4. National Animal Genetic Research Center, Beijing, PR China;5. Department of Animal Science, Oklahoma State University, Stillwater, OK, USA |
| |
Abstract: | The cells with mammary repopulating capability can achieve mammary gland morphogenesis in a suitable cellular microenvironment. Using cell surface markers of CD24, CD29 and CD49f, mouse mammary repopulating unit (MRU) has been identified in adult mammary epithelium and late embryonic mammary bud epithelium. However, embryonic MRU remains to be fully characterized at earlier mammary anlagen stage. Here we isolated discrete populations of E14.5 mouse mammary anlagen cells. Only Lin−CD24medCD29+ cell population was predicted as E14.5 MRU by examining their capacities of forming mammosphere and repopulating cleared mammary fat pad in vivo. However, when we characterized gene expressions of this E14.5 cell population by comparing with adult mouse MRU (Lin−CD24+CD29hi), the gene profiling of these two cell populations exhibited great differences. Real-time PCR and immunostaining assays uncovered that E14.5 Lin−CD24medCD29+ cell population was a heterogeneous stroma-enriched cell population. Then, limiting dilutions and single-cell assays also confirmed that E14.5 Lin−CD24medCD29+ cell population possessed low proportion of stem cells. In summary, heterogeneous Lin−CD24medCD29+ cell population exhibited mammary repopulating ability in E14.5 mammary anlagen, implying that only suitable mammary stroma could enable mammary gland morphogenesis, which relied on the interaction between rare stem cells and microenvironment. |
| |
Keywords: | Mammary gland morphogenesis Mammary repopulating units (MRU) Mammary anlagen stage Mammary stem/progenitor cell Mammary stroma |
本文献已被 ScienceDirect 等数据库收录! |
|