Crystallin synthesis in lens differentiation in cultures of neural retinal cells of chick embryos. |
| |
Authors: | M Araki M Yanagida T S Okada |
| |
Affiliation: | Institute for Biophysics, Faculty of Science, University of Kyoto, Kyoto 606, Japan |
| |
Abstract: | Dissociated cells of neural retinas of 3.5-day-old chick embryos differentiated into “lentoid bodies” within about 10–12 days when cultured in vitro. Protein synthesis of these cultured cells was studied with the use of SDS-polyacrylamide gel electrophoresis, affinity chromatography, and autoradiography combined with immunological techniques. Incorporation of [14C]leucine into total proteins, α-crystallin, and δ-crystallin was estimated after increasing times of culture up to about 30 days. Isotope incorporation into δ-crystallin was detected at 9 days, and it increased sevenfold after another 17 days. α-Crystallin was also first detected at 9 days, but its relative content reached a maximum at 12 days and then decreased gradually. The ratio of δ-crystallin synthesis to total protein synthesis increased up to 40% at 26 days, while that of α-crystallin synthesis remained 3% throughout the culture period. These results show that lens differentiation from neural retinal cells is associated with the preferential synthesis of lens crystallins, particularly of δ-crystallin. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|