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Comparative evaluation of agar dilution and broth microdilution methods for antibiotic susceptibility testing of Helicobacter cinaedi |
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| Authors: | Junko Tomida Ayako Oumi Tatsuya Okamoto Yuji Morita Akihiko Okayama Naoaki Misawa Tetsuya Hayashi Takaaki Akaike Yoshiaki Kawamura |
| Institution: | 1. Department of Microbiology, School of Pharmacy, Aichi Gakuin University, , Nagoya, 464‐8650;2. Department of Clinical Laboratory, Social Insurance Chuo General Hospital, , Tokyo, 169‐073;3. Department of Microbiology, Graduate school of Medical Sciences, Kumamoto University, , Kumamoto, 860‐8556;4. Department of Rheumatology, Infectious Diseases and Laboratory Medicine, Faculty of Medicine, University of Miyazaki, , Miyazaki, 889‐1692;5. Laboratory of Veterinary Public Health, Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, , Miyazaki, 889‐2192;6. Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, , Miyazaki, 889‐1692 Japan |
| Abstract: | The aim of this study was to establish a broth microdilution method for antimicrobial susceptibility testing of Helicobacter cinaedi and to assess the prevalence and mechanisms of fluoroquinolone resistance in Japanese clinical isolates. A broth microdilution method using modified Levinthal broth was developed and compared with the agar dilution method for testing susceptibility to ampicillin, gentamicin, tetracycline and ciprofloxacin. The minimum inhibitory concentrations obtained by these two methods were almost the same for all the antibiotics tested, demonstrating the broth microdilution method to be a suitable and reliable technique for antimicrobial susceptibility testing. A broth microdilution method for antimicrobial susceptibility test for H. cinaedi was established. This method is expected to help improve treatment. |
| Keywords: | antimicrobial susceptibility broth microdilution method Helicobacter cinaedi |