Diversion of phagosome trafficking by pathogenic Rhodococcus equi depends on mycolic acid chain length |
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| Authors: | Gitta Huth Otto Holst Jens Wohlmann Ulrike Becken Tobias Dykstra Kristina Söhl Buko Lindner John F Prescott Ulrich E Schaible Olaf Utermöhlen Albert Haas |
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| Institution: | 1. Institute for Cell Biology, University of Bonn, , Bonn, Germany;2. Research Centre Borstel, , Borstel, Germany;3. Department of Pathobiology, University of Guelph, , Guelph, ON, Canada;4. Department of Medical Microbiology, Immunology and Hygiene, University of Cologne, , Cologne, Germany;5. Center for Molecular Medicine Cologne, University of Cologne, , Cologne, Germany |
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| Abstract: | Rhodococcus equi is a close relative of Mycobacterium spp. and a facultative intracellular pathogen which arrests phagosome maturation in macrophages before the late endocytic stage. We have screened a transposon mutant library of R. equi for mutants with decreased capability to prevent phagolysosome formation. This screen yielded a mutant in the gene for β‐ketoacyl‐(acyl carrier protein)‐synthase A (KasA), a key enzyme of the long‐chain mycolic acid synthesizing FAS‐II system. The longest kasA mutant mycolic acid chains were 10 carbon units shorter than those of wild‐type bacteria. Coating of non‐pathogenic E. coli with purified wild‐type trehalose dimycolate reduced phagolysosome formation substantially which was not the case with shorter kasA mutant‐derived trehalose dimycolate. The mutant was moderately attenuated in macrophages and in a mouse infection model, but was fully cytotoxic.Whereas loss of KasA is lethal in mycobacteria, R. equi kasA mutant multiplication in broth was normal proving that long‐chain mycolic acid compounds are not necessarily required for cellular integrity and viability of the bacteria that typically produce them. This study demonstrates a central role of mycolic acid chain length in diversion of trafficking by R. equi. |
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