Mirror‐symmetric microtubule assembly and cell interactions drive lumen formation in the zebrafish neural rod |
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Authors: | Clare E Buckley Xiaoyun Ren Laura C Ward Gemma C Girdler Claudio Araya Mary J Green Brian S Clark Brian A Link Jonathan D W Clarke |
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Institution: | 1. MRC Centre for Developmental Neurobiology, King's College London, , London, UK;2. Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, , Milwaukee, WI, USA |
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Abstract: | By analysing the cellular and subcellular events that occur in the centre of the developing zebrafish neural rod, we have uncovered a novel mechanism of cell polarisation during lumen formation. Cells from each side of the neural rod interdigitate across the tissue midline. This is necessary for localisation of apical junctional proteins to the region where cells intersect the tissue midline. Cells assemble a mirror‐symmetric microtubule cytoskeleton around the tissue midline, which is necessary for the trafficking of proteins required for normal lumen formation, such as partitioning defective 3 and Rab11a to this point. This occurs in advance and is independent of the midline cell division that has been shown to have a powerful role in lumen organisation. To our knowledge, this is the first example of the initiation of apical polarisation part way along the length of a cell, rather than at a cell extremity. Although the midline division is not necessary for apical polarisation, it confers a morphogenetic advantage by efficiently eliminating cellular processes that would otherwise bridge the developing lumen. |
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Keywords: | apical polarisation lumen formation Pard3 Rab11a zebrafish |
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