hPrimpol1/CCDC111 is a human DNA primase‐polymerase required for the maintenance of genome integrity |
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Authors: | Li Wan Jiangman Lou Yisui Xia Bei Su Ting Liu Jiamin Cui Yingying Sun Huiqiang Lou Jun Huang |
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Institution: | 1. Life Sciences Institute, Zhejiang University, , Hangzhou, Zhejiang, 310058 China;2. State key laboratory of Agro‐Biotechnology, College of Biological Sciences, Agricultural University, , Beijing, 100193 China |
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Abstract: | Prim‐pol is a recently identified DNA primase‐polymerase belonging to the archaeao‐eukaryotic primase (AEP) superfamily. Here, we characterize a previously unrecognized prim‐pol in human cells, which we designate hPrimpol1 (human primase‐polymerase 1). hPrimpol1 possesses primase and DNA polymerase activities in vitro, interacts directly with RPA1 and is recruited to sites of DNA damage and stalled replication forks in an RPA1‐dependent manner. Cells depleted of hPrimpol1 display increased spontaneous DNA damage and defects in the restart of stalled replication forks. Both RPA1 binding and the primase activity of hPrimpol1 are required for its cellular function during DNA replication. Our results indicate that hPrimpol1 is a novel factor involved in the response to DNA replication stress. |
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Keywords: | hPrimpol1/CCDC111 primase‐polymerase RPA1 stalled replication fork |
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